Association of prolactin gene variants with milk production traits in Russian Red Pied cattle

A total of 125 Russian Red Pied cows were genotyped for the prolactim-related gene. The PRL-RsaI genotypes were analysed using the Polymerase chain reaction -restriction fragment length polymorphism [PCRRFLP] method. In this breed, the frequencies of alleles were as follows; A= 0.794 and B= 0.206. The frequencies of AA, AB and BB genotypes were 0.598, 0.392 and 0.01; respectively. Results showed that: BB genotype had higher milk yield than AA and AB individuals [P < 0.05]. BB genotype showed higher milk fat yield than AA and AB individuals [P < 0.05]. With respect to milk fat content [%], the AB genotype had higher levels than the AA and BB individuals [P < 0.05]. No differences between the cows of different PRL-RsaI genotypes were found in terms of milk fat yield and milk protein concentration. The results showed that the highest milk and milk fat yields were obtained by cows with the genotype PRL-RsaI BB. The results presented here demonstrate that the prolactin gene may be considered as a marker for dairy traits in cattle

Kappa-casein genotypic frequencies in Russian breeds black and red pied cattle

The present study has been designed to find out the effects of forced swimming-induced oxidative stress imposition on primary and secondary sex organs and its protection by plant extract in male Wistar strain rats. This work has been designed to find out the scientific basis of the local use of the composite extract of root of Withania somnifera, leaf of Ocimum sanctum and rhizome of Zingiber officinale by village Ayurvedic doctors to protect the health disorders in connection to strenuous physical exercise, and also to search out the potentiality of above mentioned plant products on swimming-induced oxidative damage. Forced intermittent swimming for 8 hours /day for 28 days resulted a significant elevation in the level of products of free radical i.e., thiobarbituric acid reactive substances and conjugated dienes along with significant diminution in the activities of catalase, superoxide dismutase and glutathioneStransferase in testis, prostate and seminal vesicle which were protected significantly after co-administration of methanolic extract of said plant parts in composite manner. Testicular steroidogenesis was assessed in this condition by measuring plasma levels of testosterone, which was diminished significantly in swimming group and was protected significantly by the composite extract of the plants parts. The antioxidative potency of this composite extract was compared with potent and standard antioxidant i.e., vitamin-E in forced swimming state. This herbal extract has no toxic effect on metabolic organs that has denoted here by the measurement of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities in liver and kidney. The results suggest that swimming-induced oxidative stress on male sex organs may be protected by using above mentioned medicinal plants extract